Some BSCs provide protection for experimental procedures by providing uniform, unidirectional HEPA filtered air, referred to as laminar airflow, continuously flowing over the work area. Hazardous aerosols generated during experimental procedures inside the cabinet become captured and carried by the flow of air, and trapped within HEPA filters. Air barriers provide containment by providing directional airflow from the laboratory, past the researcher, and into the cabinet via the work opening.

Principles of ContainmentĬontainment of hazardous aerosols in biological safety cabinets occurs by air barriers, physical barriers, and high-efficiency particulate air (HEPA) filtration. Discussion of another type of primary barrier, the chemical fume hood, is in Chapter 17. Some biological safety cabinets also provide a clean work environment to protect cell cultures or sterile apparatus.

Biological safety cabinets (BSC) are one type of primary barrier to contain potentially infectious research materials in order to prevent exposure of laboratory personnel and contamination of the general environment. Laboratory techniques may produce aerosols, which can contain hazardous research materials, such as infectious agents that laboratory workers could inhale. Procedures for the Proper Use of a Class II Biological Safety Cabinet.Installation and Certification of Biological Safety Cabinets.Selection of Biological Safety Cabinets.Table 16.1 - Comparison of Biological Safety Cabinet Characteristics and.Classification of Biological Safety Cabinets.This chapter describes the containment principles of biological safety cabinets (BSCs), the various classes and types of BSCs and their uses, how to select the correct type of BSC for your needs, how to get approval for installation of a BSC, and their installation and certification requirements. Laboratory Safety Manual - Chapter 16: Biological Safety Cabinets Purpose

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